Usage of Crystal Violet

Product name:Crystal Violet
CAS:548-62-9
Molecular Formula:C25N3H30Cl
Formula Weight:407.979
Description of Crystal Violet:
Crystal violet is not used as a textile dye. Instead it is used to dye paper and as a component of navy blue and black inks for printing, ball-point pens and ink-jet printers. It is also used to colourize diverse products such as fertilizers, anti-freezes, detergents, and leather jackets.
The dye is also used as a histological stain, particularly in Gram's method for classifying bacteria.
When conducting DNA gel electrophoresis, crystal violet can be used as a non-toxic DNA stain as an alternative to fluorescent, intercalating dyes such as ethidium bromide. Used in this manner it may be either incorporated into the agarose gel or applied after the electrophoresis process is finished. Used at a 0.001% concentration and allowed to stain a gel after electrophoresis for 30 minutes, it can detect as little as 16ng of DNA. Through use of a methyl orange counterstain and a more complex staining method, sensitivity can be improved further to 8 ng of DNA.[9] When crystal violet is used as an alternative to fluorescent stains, it is not necessary to use ultraviolet illumination; this has made crystal violet popular as a means of avoiding UV-induced DNA destruction when performing DNA cloning in vitro.
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